Primer Premier 6 使用手册

Copyright © 2009 by PREMIER Biosoft International. All Rights Reserved. Information in this manual may change without notice and does not represent a commitment on the part of PREMIER Biosoft International. The software described in this manual is provided by PREMIER Biosoft International under a License Agreement. The software may be used only in accordance with the terms of the agreement. PREMIER Biosoft International ("Premier") claims copyright to this program and documentation as an unpublished work. Claim of copyright does not imply waiver of Premier's other rights. This program and documentation are confidential and the property of Premier. Use, examination, reproduction, copying, decompilation, transfer, and/or disclosure to others are strictly prohibited except by express written agreement with Premier. For more information on Primer Premier and product updates, visit the PREMIER Biosoft web site at http://www.PremierBiosoft.com
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Primer Premier 6.0 Introduction to Primer Premier 1
WORKING ENVIRONMENT............................................................................................................ 3 Menus and Toolbar....................................................................................................................... 3 Main Window ................................................................................................................................ 9 The main window displays the Sequence Information Tab, Search Status Tab, Sequence View and the Search Results Tab. Sequence Information Tab ........................................................... 9 Primer Search Parameters......................................................................................................... 11 BLAST Search Parameters ........................................................................................................ 14 Template Structure Search ........................................................................................................ 19 View Cross Homology ................................................................................................................ 20 View Secondary Structures ........................................................................................................ 22 View Template Secondary Structures ........................................................................................ 23 Multiplex Results ........................................................................................................................ 24 TUTORIAL ..................................................................................................................................... 25 Create and Manage a Project...................................................................................................... 25 Create New Project .................................................................................................................... 25 Open Project............................................................................................................................... 25 Close Project .............................................................................................................................. 25 Save Project As .......................................................................................................................... 25 Save Sequence As ..................................................................................................................... 26 Open Sequence ............................................................................................................................ 27 Sequences can be opened directly from Entrez, dbSNP or from files saved on your local drive. Open Sequence from Entrez......................................................................................................27 Open Sequence From dbSNP.................................................................................................... 27 Load Multiple SNP........................................................................................................................ 29 Add SNP..................................................................................................................................... 30 Select/Unselect SNP .................................................................................................................. 30 Search Sequence ......................................................................................................................... 31 Avoid Cross Homology ............................................................................................................... 32 Interpreting Cross Homology Results......................................................................................... 36 Interpreting Human BLAST Results ........................................................................................... 37 Interpreting Microbial BLAST Results ........................................................................................ 38 Avoid Template Structures ......................................................................................................... 39 PCR Primers............................................................................................................................... 41 Primer Search Results ............................................................................................................... 43 Export Results ............................................................................................................................ 45 Evaluate predesigned primer ..................................................................................................... 46 Multiplex Primers ........................................................................................................................ 48 Generate Report......................................................................................................................... 49 Print Preview ...................................................Hale Waihona Puke Baidu.......................................................................... 50 PREFERENCES ............................................................................................................................ 51 Reaction Condition ..................................................................................................................... 51 APPLICATION PREFERENCES................................................................................................... 52
Primer Premier 6.0
Manual
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