strex STB-150 User Manual
Cell strain instrument
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Cell Stretching System
STREX Cell Strain Instrument
Model # STB-190-XY
User Manual
Cat. # STB-150
User Manual
Strex US Office
10060 Carroll Canyon Rd., Suite 100
San Diego, CA 92131
Email:
info@strexcell.com
www.strexcell.com
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Table of Contents
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Summary of Contents for strex STB-150
- Page 1 Cell Stretching System STREX Cell Strain Instrument Model # STB-190-XY User Manual Cat. # STB-150 User Manual Strex US Office 10060 Carroll Canyon Rd., Suite 100 San Diego, CA 92131 Email: info@strexcell.com www.strexcell.com...
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Page 2: Section 1: Main Components
Section 1: Main Components Cell Strain Instrument (Stretch Unit) Strain chamber brackets Each chamber is mounted on four brackets Motor Cable Cable connects to Control Unit Chamber Length Adjustment Knob: To freely rotate the knob, the power switch must be OFF. Use the knob to adjust the distance Platform of the chamber brackets such... - Page 3 Control Unit Front Panel Main Power Switch Frequency Stretch Ratio Start and Stop Button Selector (left digit) Selector (right Use to start or stop the Use upper and lower digit) stretching action. buttons adjust Use upper and lower frequency buttons to increase and decrease strain ratio.
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Page 4: Section 2: Use Of The Cell Strain Instrument
Section 2: Use of the Cell Strain Instrument Preparation of the Cell Strain Instrument Before using the Stretch Unit, sterilize the unit — especially the chamber mounting area — using ethanol-immersed swabs. System Operation Operation of the Cell Strain Instrument is very straight forward and intuitive. Below are the basic steps to use this instrument. - Page 5 5. Select the desired stretch ratio and frequency on the Control Unit. Programs are outlined in Section 3. 6. Press the START button to start the stretch cycle. During the action cycle, the green light will be illuminated. To STOP movement at any time, press the STOP button. 7.
- Page 6 ►Fibronectin Coating Preparation of fibronectin solution: 1. Dilute human or bovine fibronectin to a final concentration of 50 to 100 µg/ml in Phosphate Buffered Saline (PBS) Coating with fibronectin solution: 1. Pour 3 ml of the fibronectin solution into each strain chamber Incubate at 37 °C for more than 30 minutes Aspirate the fibronectin solution.
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Page 7: Section 3: Strain Parameters
extra-cellular matrix or coat overnight. Section 3: Strain Parameters Standard Program RIGHT Digit: Stretch Ratio Selector Digit Degree of stretch Distance 0.4mm 0.8mm 1.2mm 1.6mm 2.0mm 2.4mm 3.0mm 4.0mm LEFT Digit: Frequency Selector Digit Program Description 1cycle stretch-hold indefinitely 1cycle Stretch 0.5 seconds-hold 1 second-contract 0.5 second-end Square wave 1cycle... - Page 8 Inquire for faster speeds. Section 4: FAQ Q1: What are the characteristics of the silicone chamber? A1: The strain chamber is made from silicone elastomer consisting of polydimethylsiloxane as its major component. The chamber surface is strongly hydrophobic and cells have difficulty attaching to it;...
- Page 9 Q4: How long can cells be stretched? A4: The duration depends on cell strain and condition. However, the step motor in a STB-150 and STB-190XY is made to operate for 15 continuous minutes. The STB-140 models can run for hours to days if the cooling system is on.
- Page 10 Section 5: References 1. Effects of repetitive stretch stimulation on neonatal rat cardiocytes in vitro, K. Kada, K. Yasui, K. Naruse, and J. Toyama. Environmental Medicine, 40: 69-72, 1996. 2. Inhibitory action of repeated stretch stimulation on apoptosis in neonatal rat cardiocytes., K. Yasui, H. Shimano, K.
- Page 11 uniaxial continuous stretch, A. Sasamoto, M. Nagina. S. Kobayashi, K. Naruse, Y. Nimura, M. Sokabe, Am J Physiol Cell Physiol 288:1012-1022, 2005. 18. N-cadherin-mediated cell adhesion determines the plasticity for cell alignment in response to mechanical stretch in cultured cardiomyocytes, T. Matsuda, K. Takahashi, T. Nariai, T. Ito, T. Takatani, Y.
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Page 12: Warning / Caution
We are not responsible for damages caused by malfunctions due to combinations of equipment or software not involving Strex. We are not responsible for any incidental damage caused by the use or misuse of this product including loss of business income, interruption of business, loss of stored data, theft of machine, etc. - Page 13 Do not exceed the rating of outlets and wiring equipment. If rating is exceeded with the multiple electrical components fire may be caused due to heat generation. Do not touch the main unit or the power cable during severe weather events.
- Page 14 significantly, result in serious injury or death of the user. To ensure safe operation of the instrument, it is therefore imperative that you follow these instructions carefully. Power cable To avoid possible short circuit, shock, or fire. Only use the power cable provided with the Cell Strain Instrument. ...
- Page 15 shock. Please refrain from modifying the machine without our permission, you may be shocked injured. If you do attempt to modify the machine, the warranty on the unit is void and we will not be responsible for any performance deterioration or unit malfunction. In the case of any abnormal sound, smell, or smoke, disconnect the power immediately ...
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Page 16: Section 7: Warranty
Section 7: Warranty 1. The warranty is for one year, commencing the date the customer receives the product and includes the instrument casing, non-wearable parts, as well as, the motor and bearings. The cell culture chambers are considered consumables, B-Bridge International, Inc.